Author/Authors :
Mortaz, Esameil Chronic Respiratory Diseases Research Center and National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Department of Immunology - Shahid Beheshti University of Medical Sciences, Tehran , Gudarzi, Hoda Chronic Respiratory Diseases Research Center and National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Department of Immunology - Shahid Beheshti University of Medical Sciences, Tehran , Tabarsi, Payam Clinical Tuberculosis and Epidemiology Research Center - National Research and Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences, Tehran , Adcock, Ian M. AirwaysDisease Section - National Heart and Lung Institute - Imperial College London, London, UK , Masjedi, Mohamad Reza Chronic Respiratory Diseases Research Center and National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Department of Immunology - Shahid Beheshti University of Medical Sciences, Tehran , Jamaati, Hamid Reza Chronic Respiratory Diseases Research Center and National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Department of Immunology - Shahid Beheshti University of Medical Sciences, Tehran , Garssen, Johan Division of Pharmacology - Utrecht Institute for Pharmaceutical Sciences - Faculty of Science - Utrecht University, Utrecht, The Netherlands , Velayati, Ali Akbar Mycobacteriology Research Center (MRC) National Research Institute of Tuberculosis and lung diseases (NRITLD) - Shahid Beheshti University of Medical Sciences, Tehran , Redegeld, Frank A. Division of Pharmacology - Utrecht Institute for Pharmaceutical Sciences - Faculty of Science - Utrecht University, Utrecht, The Netherlands
Abstract :
The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. Flow cytometric analysis provides a rapid qualitative and quantitative description of multiple characteristics of individual cells. For example, it is possible to detect the cell size and granularity, aspects of DNA and RNA content and the presence of cell surface and nuclear markers which are used to characterize the phenotype of single cells.
Flow cytometry has been used for the immunophenotyping of a variety of specimens including whole blood, bone marrow, serous cavity fluids, (cerebrospinal fluid) CSF, urine and all types of body fluids. The technique has also been applied to human bronchoalveolar lavage (BAL) fluid, peritoneal fluids and blood.
In this review, we describe the current status of the application of flow cytometry as a diagnostic tool in various lung diseases.
We focus on the analysis of BAL cell composition in chronic obstructive lung disease (COPD), asthma, lung cancer, sarcoidosis, tuberculosis and idiopathic eosinophilic pneumonia (IEP).
Keywords :
Asthma , Broncho alveolar lavage (BAL) , COPD , Flow cytometry , Lung disease , Sarcoidosis
