Author/Authors :
Goudarzi, Saeid Clinical Nanomedicine Laboratory - ENT- Head & Neck Research Center - Hazrate Rasoul Akram Hospital - Iran University of Medical Sciences, Tehran , Ahmadi, Anita Biosensor Research Center Endocrinology and Metabolism Molecular-Cellular Sciences Institute - Tehran University of Medical Sciences, Tehran , Farhadi, Mohammad Clinical Nanomedicine Laboratory - ENT- Head & Neck Research Center - Hazrate Rasoul Akram Hospital - Iran University of Medical Sciences, Tehran , Kamrava, Seyed Kamran Clinical Nanomedicine Laboratory - ENT- Head & Neck Research Center - Hazrate Rasoul Akram Hospital - Iran University of Medical Sciences, Tehran , Saghafi, Shiva Immunology Asthma and Allergy Research Institute - Tehran University of Medical Sciences, Tehran , Omidfar, Kobra Biosensor Research Center Endocrinology and Metabolism Molecular-Cellular Sciences Institute - Tehran University of Medical Sciences, Tehran
Abstract :
A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb.
The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay.
In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL.
The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.
Keywords :
IgA deficiency , Immunoglobulin A , Immunochromatography , Gold nanoparticle , Screening test
