Author/Authors :
Teimoori, Ali Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Nejati, Mehrab Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Ebrahimi, Saeedeh Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Makvandi, Manoochehr Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Zandi, Milad Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , Azaran, Azarakhsh Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran
Abstract :
Non-structural protein 4 (NSP4) is a critical protein for rotavirus (RV) replication and assembly. This
protein has multiple domains and motifs that predispose its function and activity. NSP4 has a sequence
divergence in human and animal RVs. Recently, 14 genotypes (E1-E14) of NSP4 have been identified, and E1 and
E2 have been shown to be the most common genotypes in human. Methods: The gene and protein sequence of
NSP4 in RV-positive samples were inspected with the aim of NSP4 genotyping and variation analysis in viroporin
and other domains. P and G typings of RV samples were carried out by WHO primers using a semi-multiplex PCR
method. Non-typeable RV samples were amplified by conserved primers and sequenced. Results: In viroporin and
enterotoxin, conserved sequence was detected, and amino acids substitution with the same biochemical
properties was found. Conclusion: Association of NSP4 genotype with P or G genotyping G1/G9 correlates with E1
genogroups. In electrophoretyping of RV, E2 genotype had a short pattern when compared to E1.
