مرکز منطقه ای اطلاع رساني علوم و فناوري - Evolution of Susceptibility of Strains of Candida Fluconazole and Determination of Antibiotic Resistance Genes

Author/Authors :

Rezaie Keikhaie, Khadije Maternal and Fetal Health Research Center- Zabol University of Medical Sciences, Zabol , Moshtaghi, Fatemeh Department of Biology of Basic sciences- University of Zabol, Zabol , Forghani, Forough Maternal and Fetal Health Research Center- Zabol University of Medical Sciences, Zabol , Sheykhzade Asadi, Maryam Department of Microbiology- Shiraz Branch Islamic Azad University, Shiraz , Seyed Nejad, Samira Department of Microbiology- Shiraz Branch Islamic Azad University, Shiraz , Shahi, Zahra Young Researchers and Elite Club- Kerman Branch Islamic Azad University, Kerman

Abstract :

Background: Nowadays, opportunistic fungi, mainly Candida albicans, are the most joint reason of life threatening albicans isolated from patients to putrefaction in immunodeficiency sacks. Increasing resistant strains of C. albicans are an original problem in human immunodeficiency virus infectious patients. In recent years, the maximum important reason of illness and mortality, especially in hospitalized patients, iscommonin systemic fungal infections, which is caused by pathogenic yeast. In addition, antifungal treatments with conventional drugs have not been completely effective. Objectives: The aim of this study was the evolution of susceptibility of strains of Candida albicans isolated from patients to Fluconazole as well as determination of antibiotic resistance genes. Methods: The extracts of the Teucrium polium and Citrullus colocynthis were prepared using a rotary device. A total of 2 medicinal plant extracts, Teucrium polium and Citrullus colocynthis, were used to determine their minimum inhibitory concentrations (MICs) (Mg/mL) and minimum fungicidal concentrations (MFCs) against 7 clinical isolates of C. albicans. The disc diffusion susceptibility of C. albicans isolates were analyzed by standard methods. Then, reversing the fluconazole resistance gene by using polymerase chain reaction product was evaluated and electrophoresis. Data analysis was performed using software. Results: Overall, the results display that the leaf juice of C. colocynthis and T. polium had powerful antibacterial performance. Also, C. colocynthis juice exhibited higher antimicrobial performance. The ethyl acetate extracts of C. colocynthis and T. polium showed antimicrobial acting against C. albicans race. Our studies display that of all experience rate, 65.3% was resistant to nystatin, 73.3% to fluconazole. Conclusions: The existing study showed that the ethanoic juice of T. polium and C. colocynthis powder has pronounced repressive acting versus C. albicans of the get result it can be end that however ethanol in itself- has antifungal acting.