Author/Authors :
Nazifi, Narges Department of Animal Science- Faculty of Agriculture- Ferdowsi University of Mashhad, Mashhad , Mousavi, Mojtaba Department of Animal Science- Faculty of Agriculture-Lorestan University, Khorramabad , Moradi, Saeedeh Department of Animal Science- Faculty of Agriculture, University of Tabriz, Tabriz , Jaydari, Amin Department of Microbiology- Faculty of Veterinary Medicine- Lorestan University, Khorramabad , Jahandar, Mohammad Hassan Department of Agriculture- Bam Branch Islamic Azad University, Bam , Forouharmehr, Ali Department of Animal Science- Faculty of Agriculture-Lorestan University, Khorramabad
Abstract :
Leptospirosis is a widespread zoonotic disease caused by Leptospira interrogans. The conventional vaccines have some major problems.
Therefore, recombinant vaccines such as multiple-epitope vaccine are suggested. OmpL1 and lipL32 are the most important
proteins of Leptospira interrogans bacteria that canbeused in epitope prediction process to design a multiple-epitope vaccine. Hence,
in this study, the most reliable and accurate online servers were applied to predict B cell and T cell epitopes, the secondary and tertiary
structures, enzyme digestion, and antigenicity score of ompL1 and lipL32. The results showed that epitopes located at 103 - 122,
210 - 232, and 272 - 291 amino acid residues are the common epitopes between T cell (MHCI) and B cell. 288 - 308 amino acid residues
were introduced as common epitopes to stimulate both T cell (MHCI and MHCII) and B cell of ompL1 protein. In the case of LipL32
protein, 80 - 96 amino acid residues are recommended for T cell epitopes and 63-81 amino acid residues for stimulation of both B
and T cells. All the mentioned epitopes can be considered as linear epitopes in designing a recombinant vaccine based on chimeric
epitopes. It appears that these epitopes can be applied to design recombinant multiple-epitope vaccines against leptospirosis.
Keywords :
Epitope , Leptospira , Vaccine , zoonosis
