Title of article :
Determination of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC
Author/Authors :
Esmaeili, Somayeh Traditional Medicine & Materia Medica Research Center - School of Pharmacy - Shaheed Beheshti University of Medical Sciences and Health Services, Tehran , Naghibi, Farzaneh Traditional Medicine & Materia Medica Research Center - School of Pharmacy - Shaheed Beheshti University of Medical Sciences and Health Services, Tehran , Mosaddegh, Mahmoud Traditional Medicine & Materia Medica Research Center - School of Pharmacy - Shaheed Beheshti University of Medical Sciences and Health Services, Tehran , Nader, Nazli Traditional Medicine & Materia Medica Research Center - School of Pharmacy - Shaheed Beheshti University of Medical Sciences and Health Services, Tehran
Abstract :
A high performance liquid chromatography method was studied for determination of 18 β-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/ min (8-20min) and UV detection at 230 nm. The recoveries were %99.60-%102.81 with relative standard deviation between %0.01-%1.58. The relative standard deviation of the repeatability test was %2.96. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products.
Keywords :
18 β-Glycyrrhetinic acid , Glycyrrhiza glabra , High performance liquid chromatography (HPLC) , Thin layer chromatography (TLC)
Journal title :
Astroparticle Physics
