Title of article
In Vitro Effect of α-Tocopherol, Ascorbic Acid and Lycopene on Low Density Lipoprotein Glycation
Author/Authors
Ghaffari, Mohammad Ali Department of Clinical Biochemistry - School of Medicine - Ahwaz Jundishapour University of Medical Sciences, Ahwaz , Mojab, Samad Department of Clinical Biochemistry - School of Medicine - Ahwaz Jundishapour University of Medical Sciences, Ahwaz
Pages
7
From page
265
To page
271
Abstract
Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of α-tocopherol, ascorbic acid and lycopene on LDL glycation. LDL was isolated from EDTA-plasma by ultracentrifugation using a single step discontinuous gradient. LDL and glucose were incubated without and with different concentrations of lycopene, ascorbic acid and α-tocopherol. LDL glycation were estimated by sodium periodate assay. Based on this study results, α-tocopherol, ascorbic acid and lycopene decrease LDL glycation in a dose dependent manner. The electrophoretic mobility of glycated LDL decreased in presence these nutrients. These effects may be due to antioxidant properties of these nutrients and may have a role in ameliorating atherosclerotic risk of patients with diabetes mellitus.
Keywords
Low density lipoprotein (LDL) , Glycation , α-Tocopherol , Ascorbic acid , Lycopene
Journal title
Astroparticle Physics
Serial Year
2007
Record number
2481316
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