Author/Authors :
Keyhani, Elahe Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Hosseini, Shadi Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Behjati, Farkhondeh Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Rahimi, Maryam Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Taheri, Nazanin Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Khoram Khorshid, Hamidreza Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Aghakhani Moghaddam, Fatemeh Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Ghasemi, Saghar Genetics Research Center - University of Social Welfare and Rehabilitation Sciences - Tehran, Iran , Sirati, Fereidoon Cancer Institute - Tehran University of Medical Sciences - Tehran, Iran , Karimlou, Masoud Dept. of Epidemiology and Biostatistics - Tehran Medical Sciences Branch - Islamic Azad University - Tehran, Iran
Abstract :
The PI3K/AKT/mTOR pathway is known to play an important
role in regulating angiogenesis both in normal and breast cancer (BC) tissues.
PIK3CA amplification was reported in various malignancies, including approximately
10% of BC cases. The aim of this study was to identify the frequency of PIK3CA
amplification in Iranian female patients suffering from BC. Additionally, possible association
between PIK3CA amplification and P110α expression with microvascular
density (MVD) was examined.
Methods: DNA samples were extracted from paraffin embedded tumor tissue blocks
and copy number changes were evaluated by MLPA Technique. The results were analyzed
by coffalyzer software. The tissue expression of P110α and CD34 was assessed
using immunohistochemistry.
Results: Ten out of 40 samples (17.5%) showed amplification in PIK3CA gene and
22 out of 40 samples (55%) showed overexpression in P110α. For CD34, from 40
samples, 20 (50%), 15 (37.5%) and 5 (12.5%) had scores 1+, 2+ and 3+, respectively.
Conclusion: No significant association was detected between gain of PIK3CA copy
number and P110α or CD34 tissue expression
