Title of article :
Influence of Additives on Fabrication and Release from Protein Loaded Microparticles
Author/Authors :
Bhatt, Yagnesh A.P.M.C. College of Pharmaceutical Education and Research, Himmatnagar - Affiliated to Hemchandracharya North Gujarat University, Gujarat, India , Shah, Dushyant A.P.M.C. College of Pharmaceutical Education and Research, Himmatnagar - Affiliated to Hemchandracharya North Gujarat University, Gujarat, India
Abstract :
The purpose of this study was to investigate the effect of additives, poly(ethylene
glycol) (PEG) 1450, poloxamer 407, polyvinyl alcohol (PVA) and sodium chloride
in order to improve physico-chemical characteristics, encapsulation efficiency and
in vitro release of bovine serum albumin, form poly(D,L-lactic-co-glycolic acid)
(PLGA) microparticles prepared by the w/o/w solvent evaporation method. The
addition of PEG 1450 and 0.05 M NaCl changed the surface characteristics of
microparticles and also affected the encapsulation efficiency and burst release of
protein. The effect of surfactants: polyvinyl alcohol and poloxamer 407 used in the
outer water phase was investigated. The surfactant/PLGA mass ratio played an
important role in the preparation procedure of the particles. This ratio was found
to be approximately 0.5 for PVA and 5 for poloxamer 407 in order to achieve
microparticles with narrow size distribution (<70 μm) and good encapsulation
efficiency (>70%).
Keywords :
Bovine serum albumin , Encapsulation efficiency , Initial release , Modified Lowry Protein Assays , Poly (lactide-co-glycolide)
Journal title :
Astroparticle Physics
