Title of article :
Evaluation of Effect of β-Lactam Antibiotics on Suppression of Different Strains of Agrobacterium tumefaciens and on Wheat Mature Embryo Culture
Author/Authors :
Yarizade, Adel Institute of Biotechnology - Shiraz University, Shiraz , Aram, Farzaneh Institute of Biotechnology - Shiraz University, Shiraz , Niazi, Ali Institute of Biotechnology - Shiraz University, Shiraz , Ghasemi, Younes Pharmaceutical Research Center and Department of Pharmaceutical Biotechnology - Faculty of Pharmacy - Shiraz University of Medical Sciences
Abstract :
The efficiency of four β-lactam antibiotics meropenem, imipenem, ceftazidime
and ceftriaxone were tested on three strains of Agrobacterium, tumefacience
LBA4404, AGL0 and C58 and were compared with the commonly used antibiotic,
cefotaxime. Meropenem exhibited the most effective antibacterial activity against
Agrobacterium tumefacience LBA4404, AGL0 and C58. In addition to the high levels
of activity of the antibiotics meropenem and imipenem on Agrobacterium inhibition
(MIC less than 10 mg.l-l and MBC less than 150 mg.l-l), they allowed a high rate
of shoot formation and had no significant negative effects on shoot and root weights
of wheat explants. Therefore, they can be recommended for Agrobacteriummediated
transformation projects for wheat.
Keywords :
Agrobacterium tumefacience , Genetic , Impenem , Meropenem , Transformation , Wheat
Journal title :
Astroparticle Physics
