Title of article :
Anti-Alzheimer and Antioxidant Activity of Celastrus paniculatus Seed
Author/Authors :
Alam, Badrul Department of Pharmacy - Atish Dipankar University of Science and Technology, Dhaka, Bangladesh , Haque, Ekramul Department of Pharmacy - BRAC University, Dhaka, Bangladesh
Abstract :
The crude methanolic extract of the seeds of Celastrus paniculatus along with
its organic soluble fractions were tested for their possible antioxidant and antialzheirmer
(AD) activity. The extracts showed prominent DPPH free radical
scavenging activity, inhibiting activity of authentic peroxynitrite (ONOO-) and
inhibition of total reactive oxygen species (ROS) generation. In DPPH radical
scavenging assay, the EtOAc fraction showed the highest activity with a IC50
value of 25.92±1.02 μg/ml whereas aqueous fractions had no activity at all within
the tested concentration. Scavenging of the authentic ONOO- system, all
extract/fractions showed good activity and among them, EtOAc fraction had the
highest activity with a IC50 value of 15.79±0.18 μg/ml. EtOAc fraction also
showed significant (p<0.001) inhibitory activity against the total ROS generation
which was almost similar with that of the positive control Trolox (IC50 16.79±0.19
μg/ml). All extract/fractions exhibited statistically significant (p<0.001) cholinesterases
(ChEs) inhibitory effects with IC50 values ranging between 134.7-227.5 μg/ml
for AChE and 209.6-562.1 μg/ml for BChE.
Keywords :
Anti-alzheimer , Antioxidant , Cholinesterase , Celastrus paniculatus , ROS
Journal title :
Astroparticle Physics
