Author/Authors :
Cao, Gang Research Center of TCM Processing Technology - Zhejiang Chinese Medical University, Hangzhou, PR China , Feng, Jian Department of Pharmaceutics - Zhejiang Chinese Medical University, Hangzhou, P.R. China , Zhang, Yun Research Center of TCM Processing Technology - Zhejiang Chinese Medical University, Hangzhou, PR China , Cong, Xiaodong Research Center of TCM Processing Technology - Zhejiang Chinese Medical University, Hangzhou, PR China , Cai, Hao Jiangsu Key Laboratory of Chinese Medicine Processing - Nanjing University of Chinese Medicine, Nanjing, PR China , Cai, Baochang Jiangsu Key Laboratory of Chinese Medicine Processing - Nanjing University of Chinese Medicine, Nanjing, PR China
Abstract :
The purpose of this study was to develop a sensitive and fast microdialysis
coupled with high-performance liquid chromatographic (HPLC) method for
determination of 5-hydroxymethyl-2-furaldehyde (5-HMF) in free-moving rats
after i.g. administration of the aqueous extract of crud Fructus corni and its
processed products of jiuzheng pin (JZP). The concentration of 5-HMF in free-moving
rats after i.g. administration of the water extract of Fructus corni and JZP was analyzed
by microdialysis coupled with HPLC. Results demonstrated that the concentration
of 5-HMF in microdialysate was 1.4951μg/l, but higher in rat microdialysate after
i.g. administration of the aqueous extract of JZP (5.2662 μg/l). In conclusion, this
method is proved to be rapid，accurate and simple. Real-time in vivo monitoring
the concentration of 5-HMF provides the theoretical basis for further explaining the
processing mechanism of F. corni.
Keywords :
Fructus corni , 5-HMF , HPLC-DAD , Microdialysi
