Author/Authors :
Allah Abbaszadeh, Hojjat Hearing Disorders Research Center and Department of Biology and Anatomical Sciences - School of medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Tiraihi, Taki Department of Anatomical Sciences - Faculty of Medical Sciences - Tarbiat Modares University, Tehran, Iran , Sadeghi, Yousef Hearing Disorders Research Center and Department of Biology and Anatomical Sciences - School of medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Delshad, Ali Reza Department of Anatomy - Shahed University, Tehran, Iran , Sadeghizadeh, Majid Department of Genetics - School of Basic Sciences - Tarbiat Modares University, Tehran, Iran , Taheri, Taher Shefa Neurosciences Research Center - Khatam Al-Anbia Hospital, Tehran, Iran , Noori-Zadeh, Ali Department of Clinical Biochemistry - Faculty of Paramedicine - Ilam University of Medical Sciences, Ilam, Iran
Abstract :
Background: Oligodendrocyte cell death is among the important features of spinal cord injury, which appears
within 15 min and occurs intensely for 4 h after injury, in the rat spinal contusion model. Accordingly, the number
of oligodendrocytes progressively reduced within 24 h after injury. Administration of oligodendrocyte-like cells
(OLCs) into the lesion area is one of the approaches to counterbalance this condition. Methods: Bone marrow
stromal cells were transdifferentiated into neurospheres and then into neural stem cells and later were
differentiated into OLCs using triiodothyronine and transplanted into the spinal cord contusion rats. The postinjury
functional recovery was explored and compared with the control group using Basso-Beattie-Bresnahan and
narrow beam behavioral tests. At the end of 12th week, spinal cord segments T12-L1 were histomorphologically
studied by immunohistochemistry. Results: Motor improvement was more obvious during 2nd to 4th weeks and
got less prominent during 4th to 12th weeks. Histomorphometric findings indicated that cavity formation
decreased in epicenter of transplantation area in experimental groups in comparison with the control groups.
Conclusion: The findings obtained in the present study showed that OLC therapy is a potential approach in the
treatment of spinal cord traumatic injuries.
