Analytical and Physicochemical Characterization of Cefquinome Using Capillary Electrophoresis

The Capillary Electrophoresis (CE) is a very useful technique to determine and to quantify pharmaceutical materials. In this work, a capillary electrophoresis method has been developed for the determination of Cefquinome sulfate in aqueous solution and in biological media (urine, milk and plasma). The ion mobility was measured at the various pH values. The detection limit and the relative standard deviation of migration times of the peak area were determined. The influence of the pH on the separation of Cefquinome sulfate in plasma and in urine was investigated. The SDS micellar elecrokinetic chromatography (SDS-MEKC) was used for the determination of Cefquinome sulfate in water, plasma and urine. The solubility and stability of Cefquinome sulfate were considered at the different pH values. The best stability was found at pH 7.4. Also the hydrophilic/lipophilic properties of Cefquinome sulfate (partition coefficient and permeation, in vitro) were investigated. The hydrophilic properties of cefquinome affected the results; the partition coefficient was 0.01 at pH 7.4. In addition, the permeation coefficient was 1.65 at pH 7.4. Acid the dissociation constant of cefquinome was calculated using CZE. The low amount of sample required and the relatively short analyze time were the main advantages of this method.