Author/Authors :
Cuellar‑Flores, Miryam Interdisciplinary Research Laboratory - Nanostructures and Biomaterials Area - Autonomous University State of Mexico - Toluca, Mexico , Acosta‑Torres, Laura Susana Interdisciplinary Research Laboratory - Nanostructures and Biomaterials Area - Autonomous University State of Mexico - Toluca, Mexico , Martínez‑Alvarez, Omar Oral and Maxillofacial Surgery Area - Autonomous University State of Mexico - Toluca, Mexico , Sánchez‑Trocino, Benjamin Oral Public Health, National School of Higher Education - Unit Leon - National Autonomous University of Mexico, Leon , Fuente‑Hernández, Javier de la Politecnich University of Guanajuato - Cortazar - Gto - National Autonomous University of Mexico, Leon , Garcia‑Garduño, Rigoberto Faculty of Dentistry - Autonomous University State of Mexico - Toluca, Mexico , Garcia‑Contreras, Rene Interdisciplinary Research Laboratory - Nanostructures and Biomaterials Area - Autonomous University State of Mexico - Toluca, Mexico
Abstract :
Background: The surface energy of titanium (Ti) implants is very important when determining
hydrophilicity or hydrophobicity, which is vital in osseointegration. The purpose of this study was to
determine how Ti plates with an alkaline treatment (NaOH) affect the adhesion and proliferation
of human periodontal ligament fibroblasts (HPLF).
Materials and Methods: In vitro experimental study was carried out. Type 1 commercially pure
Ti plates were analyzed with atomic force microscopy to evaluate surface roughness. The plates
were treated ultrasonically with NaOH at 5 M (pH 13.7) for 45 s. HPLF previously established from
periodontal tissue was inoculated on the treated Ti plates. The adhered and proliferated viable cell
numbers were determined using the 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide
method for 60 min and 24 h, respectively. The data were analyzed using Kruskal–Wallis tests and
multiple comparisons of the Mann–Whitney U‑test, P value was fixed at 0.05.
Results: The mean roughness values equaled 0.04 μm with an almost flat surface and some grooves.
The alkaline treatment of Ti plates caused significantly (P < 0.05) more pronounced HPLF adhesion
and proliferation compared to untreated Ti plates.
Conclusion: The treatment of Ti plates with NaOH enhances cell adhesion and the proliferation
of HPLF cells. Clinically, the alkaline treatment of Ti‑based implants could be an option to improve
and accelerate osseointegration.
Keywords :
Cells , periodontal ligament , sodium hydroxide , titanium , wettability
