Title of article :
Molecular detection of mouse hepatitis virus in laboratory mouse colonies
Author/Authors :
Fallahi ، Roozbeh Department of Research, Breeding and Production of Laboratory Animals - Razi Vaccine and Serum Research Institute - Agricultural Research Education and Extension Organization (AREEO) , Abedini ، Fatemeh Department of Research, Breeding and Production of Laboratory Animals - Razi Vaccine and Serum Research Institute - Agricultural Research Education and Extension Organization (AREEO) , Shokri ، Gholam Reza Research and Development Laboratory - Razi Vaccine and Serum Research Institute - Agricultural Research Education and Extension Organization (AREEO)
Abstract :
The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.
Keywords :
Molecular detection , Mouse , Hepatitis virus
Journal title :
Iranian Journal of Veterinary Science and Technology
Journal title :
Iranian Journal of Veterinary Science and Technology
