Author/Authors :
Shi, Xiao-Hong Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Wang, Bo Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Feng, Shu-Ying Department of Obstetrics and Gynaecology - The Third People Hospital of Taiyuan City, Taiyuan, Shanxi Province, China , Guo, Jian Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Wang, Xue-Fei Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Li, Shu-Zhen Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Fengو Yong-Liang Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China , Wang, Su-Ping Department of Epidemiology - School of Public Health - Shanxi Medical University, Taiyuan, Shanxi Province, China
Abstract :
Background: Although many studies have measured HBV cccDNA molecules in Peripheral Blood Mononuclear Cells (PBMC) from
patients with active chronic hepatitis B, the current pilot study found PBMC HBV cccDNA in PBMC among HBsAg-positive mothers
and their neonates. However, the risk factor of HBV cccDNA in PBMC among HBsAg-positive pregnant female’s neonates remains
unclear.
Objectives: The aim of this study was to explore influential factors of HBVcccDNA inPBMCamongHBsAg-positive pregnant female’s
neonates.
Methods: Peripheral blood samples and clinical data were collected from 151 pregnant females, who were positive for hepatitis B
surface antigen (HBsAg) in the Third People Hospital of Taiyuan City. Blood samples from 152neonates were collected beforeimmune
prophylaxes administrationandtested forHBVmarkers,HBVDNAin serum, andHBVDNAin PBMC. Bayesian logistic regression with
Cauchy prior were used to measure the association between maternal characteristics, neonatal characteristics, and HBV cccDNA in
PBMC of neonates.
Results: Among neonates of HBsAg-positive mothers, the positive rate of cccDNA in PBMC was 4.61% (7/152). Maternal PBMC HBV
cccDNA positivity (OR = 18.411, 95%CI: 3.025 - 66.022) and neonates PBMC rcDNA positivity (OR = 13.529, 95% CI: 1.948 - 93.690) were
associated with HBV cccDNA in neonatal PBMC, respectively.
Conclusions: The study suggested that HBV cccDNA can be detected in PBMC of HBsAg-positive mother’s neonates. Maternal PBMC
HBV cccDNA positivity and neonatal PBMC rcDNA positivity are risk factors of HBV cccDNA in PBMC of neonates.
Keywords :
PBMC , HBV , HBV cccDNA , Influential Factor
