Author/Authors :
Karimi, M. H Shiraz University of Medical Sciences, Shiraz , Salek, S Shiraz University of Medical Sciences, Shiraz , Yaghobi, R Shiraz University of Medical Sciences, Shiraz , Ramzi, M Shiraz University of Medical Sciences, Shiraz , Geramizadeh, B Shiraz University of Medical Sciences, Shiraz , Kafilzadeh, F Department of Microbiology - Jahrom Branch - Islamic Azad University, Jahrom
Abstract :
Background: Cytokines are important factors determining the outcome of transplantation. The host ability
in cytokine production may be affected by cytokine genes polymorphisms.
Objective: To investigate the effect of IL-12 and TNF-α gene polymorphisms on outcome of hematopoietic
stem cell transplantation.
Methods: 90 bone marrow transplant recipients were included in this study. 30 (33%) of 90 recipients
experienced graft-versus-host disease (GVHD). IL-12 and TNF-α gene polymorphisms were evaluated by
PCR-RFLP and ARMS-PCR method, respectively.
Results: No significant difference in the distribution of IL-12 (rs3212227 +1188 A/C) and TNF-α (rs
1800629 -308 G/A) genotypes and alleles was observed between those with and without GVHD. There
was no significant association between the distribution of genotypes and the recipient sex.
Conclusion: IL-12 (rs3212227 +1188 A/C) and TNF-α (rs 1800629-308 G/A) genotypes and alleles were
not risk factors for development of GVHD.
Keywords :
Interleukin 12 , TNF-α , Hematopoietic stem cell transplantation , Graft-versus-host disease , Polymorphism
