Author/Authors :
Hossein Aghdaie, M Shiraz University of Medical Sciences, Shiraz , Azarpira, N Shiraz University of Medical Sciences, Shiraz , Shamsaeefar, A Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Motazedian, N Shiraz University of Medical Sciences, Shiraz , Kaviani, M Shiraz University of Medical Sciences, Shiraz , Esfandiari, E Shiraz University of Medical Sciences, Shiraz , Golbabapour, S Shiraz University of Medical Sciences, Shiraz , Nikeghbalian, S Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Kazemi, K Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Salahi, H Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Malek- Hosseini, S. A Department of Hepatobiliary Surgery - Shiraz University of Medical Sciences, Shiraz , Geramizadeh, B Department of Pathology - Shiraz University of Medical Sciences, Shiraz
Abstract :
Background: Hepatocyte transplantation using isolated human hepatocytes is an alternative source that
can be used for the treatment of metabolic diseases and acute liver failure as a time bridge to liver transplantation.
These cells can also be used for bioartificial liver systems and in vitro study of drug toxicity.
Objective: To determine which cold preservation solution is better maintain the liver function.
Methods: We prepared 4 cold preservation solutions made of different combination of antioxidants, chelating,
membrane protective, and anti-apoptotic agents as well as inhibitor of cyclophilin D. For hepatocyte
isolation, we used livers obtained from unused deceased donor livers and the liver of patients
with Crigler-Najjar syndrome who were candidates of partial liver transplantation. After culture and cold
preservation, the level of albumin, and urea production were measured as indices of liver functionality.
Results: We found that albumin production significantly decreased after cold preservation in solution 1.
There was no significant difference in urea production after cold preservation in solution 1 compared
with control 24 h. No significant differences in albumin production were found after cold storage in solution
2 and solution 4 compared with control 24 h. Urea production significantly decreased after cold
storage in solutions 2 and 4 compared with control 24 h. As a whole albumin and urea production were
significantly decreased after cold preservation. Although albumin and urea production were decreased
after cold preservation, but the results of albumin production of two solutions were not significantly different
from that of the control group (p=0.109 and 0.951).
Conclusion: Cold preservation of cultured human hepatocytes in solution 2 and solution 4 could maintain
the function of albumin production better than other cold preservation solutions in our experiments;
solution 1 was more effective on urea production of cultured human hepatocytes at 4 °C for 24 h. To
determine if these hepatocytes are suitable candidates for transplantation, further studies should be performed.
