Author/Authors :
Asgarbeik, Saeedeh Department of Cellular and Molecular Biology - Kharazmi University, Tehran , Angaji, Abdolhamid Department of Cellular and Molecular Biology - Kharazmi University, Tehran , Mohammad Amoli, Mahsa Metabolic Disorders Research Center - Endocrinology and Metabolism Molecular-Cellular Sciences Institute - Tehran University of Medical Sciences, Tehran , Enayati, Samaneh Endocrinology and Metabolism Research Center - Endocrinology and Metabolism Clinical Sciences Institute - Tehran University of Medical Sciences, Tehran , Bandarian, Fatemeh Personalized Medicine Research Center - Endocrinology and Metabolism Clinical Sciences Institute - Tehran University of Medical Sciences, Tehran , Nasli- Esfahani, Ensieh Diabetes Research Center - Endocrinology and Metabolism Clinical Sciences Institute - Tehran University of Medical Sciences, Tehran , Forouzanfar, Katayoon Elderly Health Research Center - Endocrinology and Metabolism Population Sciences Institute - Tehran University of Medical Sciences, Tehran , Razi, Farideh Diabetes Research Center - Endocrinology and Metabolism Clinical Sciences Institute - Tehran University of Medical Sciences, Tehran
Abstract :
Nephropathy is a common diabetes complication. ERRFI1 gene which participates in various cellular pathways has been proposed as a candidate gene in diabetic nephropathy. This study aimed to investigate the role of +808T/G polymorphism (rs377349) in ERRFI1 gene in diabetic nephropathy. In this case-control study, patients including diabetes with nephropathy (DN=104), type 2 diabetes without nephropathy (DM=100), and healthy controls (HC=106) were included. DNA was extracted from blood, and genotyping of the +808T/G polymorphism was carried out using PCR-RFLP technique. The differences for genotype and allele frequencies for +808T/G polymorphism in ERRFI1 gene between DN vs. HC and DN+DM vs. HC were significant (P<0.05) while no significant difference between DN and DM was observed. The allele frequencies were significantly different in DN vs. HC and DN+DM vs. HC in males but not in females. G allele of +808T/G polymorphism in ERRFI1 gene has no significant role in development and progression of diabetic nephropathy in diabetes patients while it is a risk allele for developing diabetes in Iranian population.
Keywords :
ERRFI1 , +808T/G , polymorphism , rs397349 , diabetic nephropathy
