Title of article :
DETECTION OF 63 KDA PROTEIN IN THE CULTURE SUPERNATANT OF LEISHMANIA TROPICA and LEISHMANIA MAJOR PROMASTIGOTES
Author/Authors :
AMIN LARI, M DEPARTMENT OF BIOCHEMISTRY - SCHOOL OF VETERINARY MEDICINE - SHIRAZ UNIVERSITY , ARDEHALI, S DEPARTMENT OF BIOCHEMISTRY - SCHOOL OF VETERINARY MEDICINE - SHIRAZ UNIVERSITY , TAHERIAN, H. DEPARTMENT OF Parasitology - SCHOOL OF MEDICINE - SHIRAZ UNIVERSITY of MEDICal SHIRAZ UNIVERSITY
Abstract :
Reference strains of Leishmania tropica and Leishmania major were recovered from cryopreservation on NNN medium, and adapted to serum-free brain-heart-infusion (BHI) medium. The cultures were centrifuged and supernatants were kept at -200°. The protein concentrations of the culture supernatants and extracts were determined and subjected to 20% SDS-PAGE with molecular weight markers. Presence of protein with MW of 63kDa was seen in samples of L. major and L. tropica with no proteins detected in the BHI-only medium. It is not known if the biological functions of the soluble 63 kDa protein are different from intact GP63 as this molecule has been a candidate for vaccine production.
Keywords :
Leishmania Tropica , LEISHMANIAN MAJOR , 63 KDA PROTEIN , CULTURE SUPERNATANT
Journal title :
Astroparticle Physics
