Title of article :
Nimodipine Protects PC12 Cells against Oxygen-Glucose Deprivation
Author/Authors :
RAHBAR-ROSHANDEL, NAHID Iran University of Medical Sciences - Razi Institute for Drug Research (RIDR) , RAZAVI, LILI North Tehran Branch Islamic Azad University - School of science - Department of Biology , TAVAKOLI-FAR, BAHAREH Iran University of Medical Science - Razi Institute of Drug Research
Abstract :
The protective effect of a L-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular viability was assessed by MTT assay. OGD-induced cell injury was significantly attenuated by nimodipine in all three treatment schedules. Application of MK801 (10mM/L), an antagonist of NMDA glutamate receptors also inhibited PC12 cell death by OGD method. Our study suggests that nimodipine has protective effects against OGD-induced neurotoxicity.
Keywords :
Nimodipine , Oxygen-glucose deprivation , PC12 cell line
Journal title :
Astroparticle Physics
