Comparative Morphologic and Morphometric Study on the Developmental Aspects of In Vitro and In Vivo Reared Echinococcus granulosus Sensu Stricto Using Differential Interference Contrast (DIC)/Nomarski and Phase Contrast Microscopy

Abstract Background: Echinococcus granulosus is a zoonotic parasite with worldwide distribution. The pre-sent study focused on comparative morphologic and morphometric observations on the devel-opmental aspects of whole body, more special the reproductive structures of in vitro reared adult worms (RAW) and in vivo reared adult worms in definitive host (AWIDH) using differential interference contrast (DIC)/Nomarski, phase contrast and routine optical microscopy. Methods: A total number of 10 in vitro and 10 in vivo reared adult worms of E. granulosus sensu stricto, G1 strain were selected. The worms were processed by Formaldehyde-Alcohol-Azocarmine-Lactophenol (FAAL). The details of morphological factors and reproductive struc-tures of each worm including 25 biometrical parameters were studied by routine optical, phase contrast and Nomarski microscopy. The details of the samples were photographed, measured and analyzed. The fine structures of the parasite including the details of cirrus sac and develop-mental stages in different strobila were more obvious observing by Nomarski microscopy. Results: The morphometric characters in the RAW and AWIDH showed that length of imma-ture proglottid, length of mature proglottid, length of suckers are larger in RAW than AWIDH worms with statistical difference. Characters in E. granulosus of RAW and AWIDH showed that total number of segments, number of mature segments and the total number of testes were greater in RAW than AWIDH worms; while only the number of mature segments was statistical-ly different is two groups. Conclusion: Application of DIC/Nomarski and phase contrast microscopy together with mor-phometric criteria are useful means for comparing the developmental aspects of in vitro and in vivo reared adults of E. granulosus.