Author/Authors :
ZEIDABADINEZHAD, Reza Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , VATANDOOST, Hassan Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , ABAI, Mohammad Reza Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , DINPARAST DJADID, Navid Graduate , RAZ, Abbasali Graduate , SEDAGHAT, Mohammad Mahdi Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , OSHAGHI, Mohamad Ali Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , RAEISI, Ahmad Graduate , ADIBI, Neda Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: Some mosquito species which belong to the Culex. pipiens complex are primary vectors for West Nile virus, Sindbis, Dirofilaria immitis, and many arboviruses. Knockdown resistance (kdr) mutations in the volt-age-gated sodium channel (VGSC) gene of Cx. pipiens that is inherited, is one of the important threats for the efficacy of pyrethroids insecticides. Knockdown resistance (kdr) mutation, L1014F, is a well-defined mecha-nism of resistance to pyrethroids and DDT in many insect species. The aim of study was to determine the mechanisms of Insecticide resistance in this species
Methods: Specimens of Cx. pipiens, the major vector of West Nile virus, were obtained in Tehran, Iran by col-lecting larvae from polluted wastewater in Qarchak of Tehran. In 2016 Insecticide susceptibility tests were per-formed according to WHO methods with deltamethrin 0.05%. We focused on determination of this point mu-tation in the VGSC gene of Cx. pipiens by Real-time PCR.
Results: Our results revealed high levels of resistance to deltamethrin 0.05%. The lethal times i.e. LT50 and LT90 for deltamethrin were 2.1530 and 8.5117 h respectively. The result of Real-time PCR confirmed the pres-ence of resistant genotype in all the members of tested population. This study is the first report on kdr geno-typing of Cx. pipiens from Tehran and our results on the VGSC gene in position L1014F confirmed the TTA to TTT nucleotide change.
Conclusion: This finding will provide a clue for management of insecticide resistance in mosquito which are vectors of arboviruses and decision for replacement of novel approach for vector control.
