Author/Authors :
Bilal Riaz, Muhammad Riphah Institute of Pharmaceutical Sciences - Riphah International University, Islamabad , Khan, Arif-ullah Riphah Institute of Pharmaceutical Sciences - Riphah International University, Islamabad , Gul Qazi, Neelam Riphah Institute of Pharmaceutical Sciences - Riphah International University, Islamabad
Abstract :
Objective(s): This study was designed to investigate various gastrointestinal effects of Manilkara
zapota (Sapodilla), exploring its anti-diarrheal, anti-secretary, anti-spasmodic, anti-ulcer and antimotility
potential.
Materials and Methods: Antidiarrheal and anti-secretary activities were investigated using castor
oil induced diarrhea and castor oil induced fluid accumulation. Isolated rabbit jejunum tissues
(antispasmodic) were employed for in vitro experiments. Antiulcer, antimotility and molecular
docking were performed using ethanol-HCl induced ulcer assay, charcoal meal transit time and Auto
Doc Vina.
Results: Mz.Cr exhibited protection against castor oil-induced diarrhea (P<0.05 vs. saline group)
and dose-dependently inhibited intestinal fluid secretions (P<0.001 vs. castor oil group). Mz.Cr
caused relaxation of spontaneous and K+ (80 Mm)-induced contractions with EC50 values of 0.11mg/
ml (0.08-0.1, n=4) and 0.16 mg/ml (0.09-0.2, n=4) respectively (*P<0.05**P<0.01 ***P<0.001). It
showed protective effect against gastric ulcers induced by ethanol-HCl (P<0.001 vs. saline group).
Mz.Cr reduced distance travelled by charcoal meal (P<0.001 vs. saline group). Plant constituents:
caffeoylquinic acid and methyl 4-O-galloylchlorogenate showed high binding affinities (E-value≥-6.5
Kcal/mol) against histaminergic H2 receptors, H+/K+ ATPase pump and voltage gated L-type calcium
channels, while possesses moderate affinities (E-value≥8 Kcal/mol) against histaminergic H1,
muscarinic M1, M3 and mu-opioid, whereas lower affinities (E-value≥9.5 Kcal/mol) vs. calmodulin,
adrenergic α1, phosphodiesterase enzyme and dopaminergic D2 receptors. Lupeol-3-acetate and
β-amyrin-3-(3’-dimethyl) butyrate observed weak affinities.
Conclusion: In present study, M. zapota is reported to exhibits anti-diarrheal, anti-secretory, antispasmodic,
anti-motility, anti-ulcer effects and computational binding affinities against gastrointestinal
targets.
Keywords :
Anti-diarrheal , Anti-secretory , Anti-spasmodic , Anti-ulcer , Manilkara zapota , Molecular docking
