Author/Authors :
Zhou, Dan Department of Natural Medicine - School of Pharmacy - Fourth Military Medical University, Xi’an, China , Yang, Qian Department of Natural Medicine - School of Pharmacy - Fourth Military Medical University, Xi’an, China , Yu, Zhe Department of Pharmaceutical Analysis - School of Pharmacy - Fourth Military Medical University, Xi’an, China , Chang, Ying Department of Pharmacy - Northwest Women and Children Hospital, Xi’an, China , Tian, Tian Department of Pharmacy - Ninth Hospital of Xi’an, Xi’an, China , Shi, Zhi-Hui Jun Bi Sha Pharmaceutical Co. Ltd. Xianyang, China , Dong, Hong-Liang Department of Pharmacy - Ninth Hospital of Xi’an, Xi’an, China , Li, Hua Department of Natural Medicine - School of Pharmacy - Fourth Military Medical University, Xi’an, China , Guo, Jun Department of Radiology - School of Stomatology - Fourth Military Medical University, Xi’an, China , Wang, Si-Wang Department of Natural Medicine - School of Pharmacy - Fourth Military Medical University, Xi’an, China
Abstract :
FeiYangchangweiyan capsule (FY capsule), a traditional Chinese medicinal preparation
consisting of three medicinal herbs, has been used to treat bacterial dysentery, acute, and
chronic gastroenteritis for several decades. In this study, a novel, convenient, accurate, and
valid method was developed by using high-performance liquid chromatography (HPLC)
coupled with diode array detection (DAD) to obtain a chromatographic fingerprint of
FeiYangchangweiyan capsule (FY capsule). Then, fourteen peaks were identified according to
MS/MS fragmentation behavior of the reference standards by using HPLC-DAD-ESI-MS/MS
analysis. At the same time, the fingerprint similarity was calculated and the contents of known
ingredients were also determined simultaneously. The result demonstrated that the HPLC
fingerprint combining similarity evaluation and quantification analysis can be successfully
applied to control the quality of FY capsule.
Keywords :
Fingerprint , FeiYangchangweiyan capsule , Traditional Chinese medicine , Quantitative analysis , HPLC-DAD-ESI-MS/MS
