Author/Authors :
Hakimizadeh, Elham Physiology-Pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Kaeidi, Ayat Department of Physiology and Pharmacology - School of Medicine, Rafsanjan University of Medical Sciences , Taghipour, Zahra Department of Anatomy - Rafsanjan University of Medical Sciences, Rafsanjan , Mehrzadi, Saeed Razi Drug Research Center - Iran University of Medical Sciences, Tehran , Allahtavakoli, Mohammad Physiology-Pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Shamsizadeh, Ali Department of Physiology and Pharmacology - School of Medicine, Rafsanjan University of Medical Sciences , Bazmandegan, Gholamreza Physiology-Pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences , Hassanshahi, Jalal Department of Physiology and Pharmacology - School of Medicine, Rafsanjan University of Medical Sciences , Aflatoonian, Mohammad Reza Research Center for Tropical and Infectious Diseases - Kerman University of Medical Sciences, Kerman , Fatemi, Iman Research Center for Tropical and Infectious Diseases - Kerman University of Medical Sciences, Kerman - Physiology-Pharmacology Research Center - Research Institute of Basic Medical Sciences - Rafsanjan University of Medical Sciences
Abstract :
Objective(s): Ceftriaxone (Cef), a beta-lactam antibiotic, is accompanied by antioxidant and antiinflammatory
properties. It has been shown that Cef has beneficial effects on Alzheimer’s disease. In
the current investigation, the effect of Cef in a mice model of aging was investigated.
Materials and Methods: Forty male mice were equally aliquoted into four groups as follows: Control (as
healthy normal animals), D-galactose (DG) group (treated with 500 mg/kg/day DG for 6 weeks), DG + Cef
group (treated with DG plus Cef 200 mg/kg/day for 6 weeks), and Cef group (treated with Cef 200 mg/kg/
day for 6 weeks). A battery of behavioral tests was done to evaluate age-related neurocognitive changes. The
activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as the
level of malondialdehyde (MDA) in the brain, were measured by biochemical methods. Also, to determine
the brain damage, histopathological alterations in the hippocampus were measured using hematoxylin and
eosin (H&E) staining.
Results: Our results indicate that neurobehavioral dysfunctions of DG can be prevented by coadministration
of Cef. We also found that Cef increases the activity of SOD, GPx, and CAT as well as
decreasing the level of MDA in the brain of aged mice.
Conclusion: Based on our findings, Cef declines neurocognitive dysfunctions in the DG-induced model
of aging, possibly through its antioxidative properties.
Keywords :
Aging , Ceftriaxone , D-galactose , Mice , Oxidative stress
