Title of article :
Cryopreservation of Echinococcus granulosus Protoscoleces
Author/Authors :
BADIRZADEH, Alireza Department of Parasitology and Mycology - School of Medicine - Iran University of Medical Sciences, Tehran , RAEGHI, Saber Department of Laboratory Sciences - Maragheh University of Medical Sciences, Maragheh , FALLAH-OMRANI, Vahid Department of Biotechnology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , ROUHANI, Soheila Department of Medical Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Background: The main objective of the current study was to investigate on the cryopreservation of protoscole-ces of Echinococcus granulosus, a causative agent of cystic hydatidosis in man.
Methods: This study was conducted on isolated protoscoleces from hydatid cysts infected livers collected from slaughterhouse of Tehran, Iran in 2016. Viability of protoscoleces was evaluated by dye test. Cryopreservation of isolated protoscoleces in the presence of Dimethylsulfoxide (DMSO) and glycerol using a three-step cooling pro-tocol involving an initial period at -20 °C, -80 °C and liquid nitrogen was performed.
Results: The mean viability rate of 10% DMSO and 15% glycerol were 9% and 8% respectively. The protosco-leces of Echinococcus granulosus have been successfully thawed and recovered after 6 months storage in liquid nitro-gen.
Conclusion: Cryopreservation method needs to be improved for each species of helminthes and can be useful for other immunological and laboratorial studies.
Keywords :
Echinococcus granulosus , Cryopreservation , Dimethylsulfoxide (DMSO) , Glycerol , Cystic hydatidosis , Iran
Journal title :
Astroparticle Physics
