Title of article :
The effects of synthetic orally administrated insulin nanoparticles in comparison to injectable insulin on the renal function markers of type 1- diabetic rats
Author/Authors :
Kheiripour, Nejat Research Center for Biochemistry and Nutrition in Metabolic Diseases - Kashan University of Medical Sciences , Alipoor, Behnam Department of Laboratory Sciences - Faculty of Paramedicine - Yasuj University of Medical Sciences , Ranjbar, Akram Toxicology and Pharmacology Department - School of Pharmacy - Hamadan University of Medical Sciences , Pourfarjam, Yasin Department of Chemistry - University of Cincinnati - Cincinnati - OH, United States of America , Kazemi Najafabadi, Farzaneh Toxicology and Pharmacology Department - School of Pharmacy - Hamadan University of Medical Sciences , Dehkhodaei, Narges Toxicology and Pharmacology Department - School of Pharmacy - Hamadan University of Medical Sciences , Farhadiannezhad, Masoumeh Student Research Committee - Abadan School of Medical Sciences, Abadan , Ghasemi, Hassan Department of Clinical Biochemistry - Abadan Faculty of Medical Sciences, Abadan
Abstract :
Objective(s): Injectable insulin is the most widely used therapy in patients with type 1 diabetes which
has several disadvantages. The present study was aimed to evaluate the efficacy of injectable insulin on
diabetes mellitus-related complications in comparison to orally encapsulated insulin nanoparticles.
Materials and Methods: This study involved 42 Wistar rats separated into 5 groups, including control
(C), diabetic control (D), diabetic receiving regular insulin (INS), diabetic receiving encapsulated
insulin nanoparticle (INP), and diabetic receiving chitosan for two months. Biochemical parameters in
serum and urine were measured using spectrophotometric or ELISA methods. mRNA levels of kidney
injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) were evaluated
using quantitative PCR.
Results: There were no significant differences between the two forms of insulin in controlling the
glycemic condition (P-value>0.05), but oral INP was more effective in correcting diabetic dyslipidemia
in comparison to injectable insulin (P-value<0.05). Urine volume and creatinine excretion were
significantly modulated by insulin and oral INP in diabetic groups (P-value<0.05), although the effects
of INP on the modulation of execration of urea, acid uric, and albumin was more dramatic. Oral INP
caused a significant decrease in urine concentration of KIM-1 and NGAL as well as expression of KIM-1
in renal tissue (P-value<0.05).
Conclusion: Our results suggested that oral INP is more effective than injectable insulin in modulation
of urine and serum diabetic-related parameters.
Keywords :
Chitosan , Diabetes mellitus , Insulin nanoparticle , Kidney injury molecule 1 , Neutrophil gelatinaseassociated lipocalin protein
Journal title :
Astroparticle Physics
