Author/Authors :
FARZANEHPOUR, Mahdieh Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , MUHAMMADNEJAD, Ahad Tehran University of Medical Sciences, Tehran , AKHAVAN, Setareh Department of Gynecology/Oncology - Imam Khomeini Hospital Complex - Valiasr Hospital - Tehran University of Medical Sciences, Tehran , EMAMI RAZAVI, Amir Nader Tehran University of Medical Sciences, Tehran , JALILVAND, Somayeh Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , SALIMI, Vahid Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , FAGHIHLOO, Ebrahim Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , KAKAVANDI, Ehsan Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , FARAHMAND, Mohammad Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , SHAYESTEHPOUR, Mohammad Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , BABAKHANI, Farzad Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , MOKHTARI AZAD, Talat Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: High-risk (HR) Human papillomaviruses (HPVs) are known as the main factors implicated in the pathogenesis of cervical preinvasive and invasive lesions. Therefore, the presence or absence of HR-HPV can be followed for the prognosis of low-grade and high-grade squamous intraepithelial lesions. Since the overex-pression of p16INK4a protein depends on the presence of transcriptionally-active HPV, and due to its availabil-ity and simple interpretation, it may be considered as a proper marker to diagnose cervical cancer.
Methods: An immunohistochemical analysis of p16INK4a was performed in 72 cervical tissue specimens at Imam Khomeini Complex Hospital (Tehran, Iran) from 2016 to 2018. The performance parameters were calcu-lated and compared using receiving operating characteristics curve (ROC) details.
Results: p16INK4a is significantly up-regulated in the cervical cancer samples in comparison with that in nor-mal samples. Moreover, the ROC data showed the potential ability of p16INK4a under determined conditions as a diagnostic marker for CIN 2-3 staging and invasive cervical cancer. The molecular typing disclosed the at-tendance of HPV DNA in 44.4% of cases (32/72) with a predominance of HPV type 16.
Conclusion: The molecular biomarker p16INK4a can be a good candidate for the early diagnosis and progno-sis of cervical cancer in HPV-infected patients. Considering the increase in the expression level of p16INK4a in cancer and precancer tissues, p16INK4a may be used for early detection of cervical cancer.
