Author/Authors :
Ravanshad, M Department of Medical Virology - Faculty of Medicine - Tarbiat Modarres University, Tehran , Roustai, MH Department of Medical Virology - Faculty of Medicine - Tarbiat Modarres University, Tehran , Mostafaee, A Department of Immunology - Faculty of Medicine - Kermanshah University, Kermanshah , Sabahi, F Department of Medical Virology - Faculty of Medicine - Tarbiat Modarres University, Tehran , Soleimanjahi, H Department of Medical Virology - Faculty of Medicine - Tarbiat Modarres University, Tehran , Ziyaeyan, M Department of Medical Virology - Faculty of Medicine - Tarbiat Modarres University, Tehran
Abstract :
An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies
