Association of bcsA and draE genes with biofilm formation on urinary catheter in uropathogenic Escherichia coli isolates

The production of biofilm by E. coli strain can cause serious problem for human health. Catheter is considered to be a suitable place for colonization of microorganisms, so that in 5-10% of patients who used the catheter for only one day and also in all patients who used it for over 28 days, the colonization of bacteria is observed on catheter. The aim of this study was to compare biofilm formation in E. coli and its relationship with pathogenic factors. In this study 144 isolates of uropathogenic E.coli were used in order to compare their potential of biofilm formation on urinary catheter. In order to do this, a piece of the catheter was sliced and put in the tubes containing E. coli suspension, as well as in broth medium (as witness). After incubation at 37oC for 24 hours, the number of live bacteria involved in biofilm formation was counted by surface cultivation method. The result showed that among the 144 studied E.coli isolates, 130 (89.7%) had cellulose synthesized gene (bcsA), and 22(15.2%) had Dr adhesine gene (draE). There was a significant relationship between bcsA gene and biofilm formation potential on urinary catheter.