Phytochemical analysis of some herbal extracts and assessed their antimicrobial effect on the bacterial isolates from urinary tract infected patients

Urinary tract infection is one of the most common infections among women. The diseases is responsible for many visits to physicians and isolating of antibiotic resistance bacteria from infected women encouraging the scientist to isolates bacteria and evaluate the effect of herbal plants on the isolates. For this purpose 100 urine samples were collected from women who admitted in Iranpour hospital, Omideye. The samples were cultivated on Blood agar, MacConkey as well as Eosin methylene blue agar. Then they were identified using biochemical tests and verified using Api 20E. The WBC of the positive culture was analyzed and antibiotic susceptibility of the isolates was checked. Then effect of 3 medicinal plants, including: Myrtus communis, Achillea millefolium and Citrullus colocynthis were evaluated on the islaets. Furthermore, phytochemical screening of the medicinal plants was evaluated and MIC for the best plant was checked. Finally, identification of phytochemical compounds was performed using HPLC. Out of all, 9 isolates were detected and all of them were belonging to Enterobactereacae family. Most of the isolates were resistant to cephalothin and the most effective drug was ciprofloxcacin. Among the water, ethanolic and acetonic extract the most effective were ethanol extract of Myrtus communis. The extracts showed different compound including: tannins, glycosides, saponins, alkaloids, flavonoids, quinone and phenoloic compounds with antibacterial capability. In addition presence of phenolic compound verified using HPLC.