Title of article :
Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran
Author/Authors :
Hosseini-Chegeni ، A. Department of Plant Protection - Faculty of Agriculture - Lorestan University , Tavakoli ، M. Lorestan Agricultural and Natural Resources Research Center - Agricultural Research, Education and Extension Organization (AREEO) , Goudarzi ، Gh. Department of Medical Microbiology - Lorestan University of Medical Sciences , Telmadarraiy ، Z. Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences , Sharifdini ، M. Department of Medical Parasitology and Mycology - School of Medicine - Guilan University of Medical Sciences , Faghihi ، F. Cellular and Molecular Research Center - Iran University of Medical Sciences , Ghanbari ، M.K. Department of National Program of Zoonotic Disease - School of Health Management and Information Sciences - Iran University of Medical Sciences
Abstract :
The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.
Keywords :
Anaplasma , Phylogenetic tree , msp4 , Iran , Tick
Journal title :
Archives of Razi Institute
