Author/Authors :
Mahmoudi, I Research Unit "Bioconservation and Valorization of Agro-Food Products" (UR 13AGR 02) - School of Food Industries of Tunis (ESIAT), Tunis, Tunisia , Ben Moussa, O Research Unit "Bioconservation and Valorization of Agro-Food Products" (UR 13AGR 02) - School of Food Industries of Tunis (ESIAT), Tunis, Tunisia , Khaldi, T.E Research Unit "Animal and Functionalities of Animal Products" (URAFPA) - University of Lorraine Team "Proteolysis & Biofunctionalities of Proteins & Peptides" (PB2P), Vandoeuvre-les-Nancy, France , Kebouchi, M Research Unit "Animal and Functionalities of Animal Products" (URAFPA) - University of Lorraine Team "Proteolysis & Biofunctionalities of Proteins & Peptides" (PB2P), Vandoeuvre-les-Nancy, France , Soligot-Hognon, C Research Unit "Animal and Functionalities of Animal Products" (URAFPA) - University of Lorraine Team "Proteolysis & Biofunctionalities of Proteins & Peptides" (PB2P), Vandoeuvre-les-Nancy, France , Leroux, Y Research Unit "Animal and Functionalities of Animal Products" (URAFPA) - University of Lorraine Team "Proteolysis & Biofunctionalities of Proteins & Peptides" (PB2P), Vandoeuvre-les-Nancy, France , Hassouna, M Research Unit "Bioconservation and Valorization of Agro-Food Products" (UR 13AGR 02) - School of Food Industries of Tunis (ESIAT), Tunis, Tunisia
Abstract :
Four hundred strains isolated from Tunisian sheep and goat raw milks were initially
screened for their ability to survive the GastroIntestinal Tract (GIT). Forty-three
among the four hundred bacteria were resistant to pepsin, pH 2, pancreatin and bile
salts at 0.3%, even after 5 hours of incubation. Identification using 16S rRNA gene
sequencing was established and we obtained as a species Lactobacillus plantarum (29
isolates from sheep milk and 11 from goat milk) and Lactobacillus pentosus (2 isolates
from sheep milk and 1 from goat milk). We showed the ability for auto-aggregation
and/or hydrophobicity properties. Finally, both M63 and C78 strains showed an
important level of adhesion to three intestinal epithelial cells Caco-2 TC7, HT29-MTX,
and HT29-CL.16E. Taken together, these properties allow the lactobacilli strains to be
considered promising beneficial strains for developing functional foods for consumers.
Farsi abstract :
در ابتداي آزمايش، چهارصد ريسه جدا شده از شير گوسفند و بز تونسي از نظر توانايي بقا در دستگاه گوارش غربال شد. از ميان آنها، مقاومت 43 باكتري به پپسين، 2=pH پانكراتين و نمك صفرا در حد ٪3 حتي بعد از 5 ساعت قابل مشاهده بود. شناسايي آنها با استفاده از توالي ژني 16S rRNA انجام شد
و ما به گونه ( Lactobacillus plantarun) (29 جدايه از شير گوسفند و 11 جدايه از شير بز) و Lactobacillus pentOSUS(2 مورد از شير گوسفند و 1 مورد از شير بز) دست يافتيم. ما توانايي
خود انعقادي و/يا آب گريزي( hydrophobicity) را نشان داديم. بالاخره، هر دو ريسه M63و C78 سطح مهمي از چسبندگي به سه سلول اپيتليال دستگاه گوارشي شامل
HT29 - Caco -2 TC7 MTX و HT29- CL . 16E
را بروز دادند. رويهم رفته، بر اساس اين خواص، مي توان ريسه هاي لاكتوباسيل را ريسه هايي با فوايد اميد بخش براي توليد غذا هاي كاربردي (functional foods) براي مصرف كننده قلمداد كرد.
Keywords :
Lactobacilli , Hydrophobicity , Functional foods , Auto-aggregation
