Other language title :
تاثير مكمل سازي جيره با اسيد لينولئيك كنژوگه بر بيان ژن سايتوكين هاي پيش و ضد التهابي در رحم گاوهاي شيري هلشتاين
Title of article :
Effects of Dietary Supplementation of Conjugated Linoleic Acid on Pro- and Anti-Inflammatory Cytokines Gene Expression in Uterus of Holstein Dairy Cows
Author/Authors :
Abolghasemi, A Department of Animal Science - Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Islamic Republic of Iran , Ansari Pirsaraei, Z Department of Animal Science - Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Islamic Republic of Iran , Dirandeh, E Department of Animal Science - Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Islamic Republic of Iran , Shohreh, B Department of Animal Science - Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Islamic Republic of Iran
Abstract :
After parturition, inflammation of the reproductive tract in cows is common because of
bacterial contamination of the uterine lumen. To investigate the beneficial effects of
dietary Conjugated Linoleic Acid (CLA) supplementation on health status mediated by
change in pro- and anti-inflammatory cytokine genes expression, endometrial samples
were collected from Holstein cows with similar parturition date and reproductive records.
From day 21 before calving to day 42 after calving, cows were fed isonitrogenous,
isocaloric, and isolipidic diets that differed only in the source of fats. Cows were fed diets
supplemented with palm oil as control group (saturated FA; n= 8), and CLA for the
treatments, and the rate of each fat in any diet was 75 g d-1. CLA-supplemented diet was
provided with a mixture of trans-10, cis-12 CLA and cis-9, trans-11 CLA isomer. Rumen
protected CLA provided 10 g d-1 each of trans-10, cis-12 CLA and cis-9, trans-11 CLA
isomers. Uterine endometrial biopsies were collected at days 21 and 42 after calving and
were prepared to determine pro-inflammatory [including Tumor Necrosis Factor-α
(TNFα), InterLeukins (IL-1, IL-6, IL-8) and InterFeron-gamma (IFN-γ)] and antiinflammatory
[interleukin 10 (IL-10)] cytokine genes expression. Results showed that
dietary CLA supplementation decreased the expression of IL-1 and IL-8 at days 21
(respectively, 1.8 and 3.9 fold) and 42 (respectively, 4 and 104 fold) postpartum and
increased expression of IL-10 at days 21 and 42 (respectively, 9.7 and 2.5 fold). The TNFα
expression significantly decreased in day 21 in CLA groups compared with palm (2.5
fold). There was no difference between groups for IL-6 expression. IFN-γ expression
decreased in day 21 (3.0 fold) and, conversely, increased in day 42 (2.5 fold) in CLA group
compared to palm. Our results showed that, during transition period, dietary
supplementation with CLA reduced inflammatory processes via inhibiting proinflammatory
cytokines and stimulating anti-inflammatory cytokines.
Farsi abstract :
براي بررسي اثرات اسيد لينولئيك مزدوج (CLA) خوراكي بر بيان ژن هاي سايتوكين هاي پيش التهابي و ضد التهابي در اندومتريوم، از گاوهاي هلشتاين كه از نظر تاريخ زايش، سوابق توليد مثلي و ركورد پيشين مشابه بودند نمونه برداري شد .گاوها از 21 روز پيش از زايش تا 42 روز پس از زايش با جيره ي ايزونيتروژنوس، ايزو كالريك و ايزوليپيديك كه تنها از لحاظ منبع چربي متفاوت بود تغذيه شدند .از نظر چربي جيره ي تيمار شاهد داراي 75 گرم روغن عبوري پالم و جيره ي آزمايشي داراي 75 گرم CLAبود .
CLAداراي 10 درصد از هر يك از ايزومر هاي 10-ترانس، 12 سيس و 9سيس، 11 ترانس بود . مكمل نمونه هاي رحم كه در روزهاي 21 و 42 پس از زايش به روش بيوپسي جمع آوري شدند، براي ارزيابي ژن ) و - اينترفرون گاما (INF-y IL ) 8 - IL6 - ILI هاي سايتوكين پيش التهابي از جمله اينترلوكين ها ) سايتوكين ضد التهابي اينترلوكين 10) 10 (ILمورد بررسي قرار گرفتند. نتايج نشان دادند كه تغذيه با CLA موجب كاهش ژن هاي 1IL-و 6 IL-و افزايش بيان ژن 10 IL-در هر دو روز 21 و 42 پس از زايش شد .با توجه به نتايج به دست آمده، تغذيه CLAدر دوره ي پس از زايمان با كاهش سايتوكين هاي پيش التهابي، در پي كاهش التهاب، موجب بهبود عملكرد رحم مي شوند.
Keywords :
TNFα expression , Palm oil , Inflammation , Endometrium
