Efficiency of Green Synthesized Silver Nanoparticles with Sweet Orange, Citrus sinensis (L.) (Rutaceae, Sapindales) against Tribolium confusum Duval. (Coleoptera, Tenebrionidae)

The post-harvest damage caused by several pests is a major problem in stored grain product industry. Recently, synthesizing NanoParticles (NPs) with plant extracts act as an alternative approach in reduction of this damage. An attempt was made to synthesize and test the mortality effect of green synthesized silver NanoParticles (AgNPs) with peels extract of sweet orange, Citrus sinensis (L.) (Rutaceae, Sapindales), against the adult tenebrionid beetle Tribolium confusum (Duval). The synthesized nanoparticles were characterized with UV-visible spectroscopy, X-ray Diffraction, and Transmission Electron Microscopy. Five bioassays were conducted by filter-paper residue and feeding methods with different concentrations of both green synthesizing and commercial AgNPs. Results showed that citrus extract by itself was not toxic against beetles, however, the green AgNPs showed 83-77% mortality in filter-paper residue and feeding methods. The impact of commercial AgNPs in both methods led to 75 and 40% mortality. The lowest LC50 and LT50 for green synthesized AgNPs were recorded (30.62 ppm and 9.92 day) for filter-paper residue tests. According to the results, the green silver nanoparticles, showed more efficiency than the commercial ones. This provides an evidence that green synthesized AgNPs have a great potential as an alternative option in stored products pest management.