Study of Genetic Variation in Three Species of Hypnea J.V. Lamouroux (Cystocloniaceae, Gigartinales) Species Using ISSR Markers

Hypnea species were found in southern coasts of Iran. This genus is an important red alga which comprises about 53 species worldwide and has a wide geographical distribution on the tropical shores around the world. In Iran, about 10 species of this genus have been reported from the subtidal zone of the Persian Gulf and Gulf of Oman coasts. The present study considers the assessment of genetic diversity of 10 populations of 3 species of Hypnea by using 6 ISSR primers. Genetic diversity parameters were determined among populations. The genetic divergence of the studied populations was checked by Neighbor-Joining (NJ) and Principal component analysis (PCA). Genetic differentiation of the studied species and populations was studied by AMOVA (Analysis of molecular variance) test. The Mantel test was performed to study the association between molecular distance and geographical distance of the studied populations. Grouping of the populations by NJ clustering separated the studied species in 2 distinct clusters. The most populations of H. musciformis formed a separate cluster and were placed far from the other species. H. ecklonii and H. cornuta showed some degree of relationship and were placed close to each other. AMOVA test showed significant genetic difference among populations. The Mantel test did not show a correlation between the genetic distance and geographical distance of these populations