Author/Authors :
Mohtasham Amiri, Zahra Guilan Road Trauma Research Center - Department of Community Medicine - School of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Tanideh, Nader Stem Cell and Transgenic Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Seddighi, Anahita Department of Genetics - Tehran Branch Islamic Azad University, Tehran, Iran , Mokhtari, Maral Department of Pathology - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , Amini, Masood Minimal Invasive Laparascopy Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Shakouri Partovi, Alborz Guilan Road Trauma Research Center - Department of Community Medicine - School of Medicine - Guilan University of Medical Sciences, Rasht, Iran , Manafi, Amir Iran University of Medical Sciences, Tehran, Iran , Hashemi, Sara Burn and Wound Healing Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Mehrabani, Davood Stem Cell and Transgenic Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
BACKGROUND Burn is the most devastating condition in emergency medicine leading to chronic disabilities. This study aimed to compare the effect of Lithospermum officinale, silver sulfadiazine and alpha ointments on healing of burn wounds in rat. METHODS Ninety-five rats were divided into 5 groups. Group 1 just underwent burn injury, and groups 2-5 received alpha ointment, silver sulfadiazine (SSD), gel base and L. officinale extract, respectively. A hot plate was used for induction of a standard 3rd degree burn wound. Burn wounds were macroscopically and microscopically evaluated on days 7th, 14th and 21st after burn induction. RESULTS A decrease in the number of inflammatory cells was noted when L. officinale and SSD were applied while the most inflammatory response was seen after administration of alpha ointment. The number of macrophages alone decreased after burn injury, while the frequency was the most when L. officinale and alpha ointment were applied. Re-epithelialization, angiogenesis and formation of granulation tissue were the best in relation to L. officinale and alpha ointment while, the worst results belonged to burn injury group and SSD regarding granulation tissue formation. Considering histological assessment, the best results were observed for scoring of inflammation, re-epithelialization, angiogenesis, formation of granulation tissue and number of macrophage when L. officinale and alpha ointment were used after burn injury. CONCLUSION It can be concluded that topical application of L. officinale as a non-toxic, inexpensive and easy to produce herbal can lead to a rapid epithelialization and wound healing and these findings can be added to the literature on burn wound healing.
Keywords :
Burn , Lithospermum officinale , Silver sulfadiazine , Alpha ointment , Wound healing , Rat
