Title of article
Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)
Author/Authors
Chow, Seinen National Research Institute of Fisheries Science, Japan , Suzuki, Nobuaki National Research Institute of Far Seas Fisheries, Shizuoka , Nakamura, Yoji National Research Institute of Fisheries Science, Japan , Yasuike, Motoshige National Research Institute of Fisheries Science, Japan , Saitoh, Kenji National Research Institute of Fisheries Science, Japan , Yanagimoto, Takashi National Research Institute of Fisheries Science, Japan
Pages
7
From page
53
To page
59
Abstract
Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed. These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was investigated using distant fish species.
Keywords
Thunnus orientalis , Four calmodulin genes , Locus-specific primers , Cross-species amplification , DNA marker
Serial Year
2017
Record number
2497366
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