The Effect of Endurance Training and Purslane Seed Consumption on NF-B and CRP in the Heart Tissue of Rats Exposed to Oxidative Damage Induced by H2O2

Background: Oxidative stress is a state of imbalance in capacity to neutralize the free radicals and its production. Objectives: The present study aimed to investigate the effect of endurance training and purslane seed consumption on NF-B and CRP in the heart tissue of rats poisoned by H2O2. Methods: Sixty-four adult male rats were randomly assigned into eight groups of eight rats (1) control, (2) endurance training, (3) endurance training with 50 mg/kg/day supplementation of purslane seed, (4) endurance training with 200 mg/kg/day supplementation of purslane seed, (5) endurance training with400mg/kg/day supplementation of purslane seed, (6)50mg/kg/day supplementation of purslane seed, (7) 200 mg/kg/day supplementation of purslane seed, and (8) 400 mg/kg/day supplementation of purslane seed. During eight weeks groups one through eight received 1 mmol/kg H2O2 three times per week intraperitoneally; groups two through four ran on a treadmill three sessions per week, and groups two through eight received purslane seed intraperitoneally daily. Two-way ANOVA and the Bonferroni post hoc tests were used for statistical data analysis (P0.05). Results: Endurance training significantly increased CRP protein levels (P = 0.001), however, the purslane seed consumption significantly reducedCRPprotein levels (P=0.001), andendurance training with purslane seed extract consumptionhadinteractive effects on reduction of CRP protein levels (P = 0.0.001). Endurance training had no significant effect on NF-B protein levels (P = 0.172), however, the purslane seed consumption had a significant effect on the reduction of NF-B (P = 0.0.001), and endurance training with purslane seed consumption had interactive effects on reduction of NF-B (P = 0.0.001). Conclusions: It appears that endurance training with concurrent consumption of purslane seed has interactive effects on the reduction of NF-B and CRP in the heart tissue of rats poisoned by H2O2.