Author/Authors :
Khosravi, Ayyoob Department of Molecular Medicine - Faculty of Advanced Medical Technologies - Golestan University of Medical Sciences, Gorgan , Kokabi, Fariba Golestan University of Medical Sciences, Gorgan , Behzadi, Ramezan Pasteur Institute of Iran, Amol , Asadi, Jahanbakhsh Golestan University of Medical Sciences, Gorgan
Abstract :
Background and objectives: Modeling cancer in vivo is a very important tool to
investigate cancer pathogenesis and molecular mechanisms involved in cancer
progression. Laboratory mice are the most common animal used for rebuilding human
cancer in vivo. Cancer stem cells (CSCs) are the main reason of failure in cancer therapy
because of tumor relapse and metastasis. Isolation of cancer stem cells helps us to study
their function and behavior. In the current study we separate cancer stem-like cells using
sphere formation assay then investigate their tumorigenicity in xenograft tumor model.
Methods: YM1 cancer cells were cultured in serum-free media (SFM) in low adherent
culture dishes for enrichment of cancer stem cells. The resulting spheres containing
cancer stem-like cells were dissociated into single cells and were injected into the dorsal
flank of B6 nude mice.
Results: A few days after injection, subcutaneous tumors formed. The growth curves of
the resulting tumors were plotted using their weekly recorded lengths. The tumors'
volume and weight were measured. The size of resulting tumors was appropriate to the
number of cells injected. Pathological analysis confirmed esophageal origin of the
resulting tumors.
Conclusion: Using laboratory mice models is a practical modeling system that provides
us investigation of human tumors pathogenesis in vivo.
