Identification of DR75-RS11090 Gene Encoding GGDEF Domains, and its Role in Enterococcus faecalis

background: enterococcus faecalis is considered the primary pathogenic bacteria in failed root canal therapy due to its excellent biofilm formation ability. the second messenger cyclic dimeric (3’→5’) gmp (c-di-gmp) is involved in bacterial physiological pro- cesses, including biofilm formation. c-di-gmp is formed by diguanylate cyclases with the ggdef domain. objectives: the current study aimed at investigating the function of dr75-rs11090 in e. faecalis. methods: in the current experimental study, gene encoding ggdef domains in e. faecalis were analyzed through national center for biotechnology information (ncbi) database. enterococcus faecalis strains were cultured in brain-heart-infusion (bhi) broth at 37°c. growth curves of e. faecalis were measured in an automatic microplate reader. biofilms were fixed with 2% glutaraldehyde and scanned under the transmission electron microscopy. acid and water-insoluble polysaccharide productions were also detected by the precise digital acidometer and anthrone method. results: it was found that dr75-rs11090 gene was predicted to encode ggdef domain in e. faecalis. the current study observed promoting effects of dr75-rs11090 on e. faecalis biofilm formation, growth, acid production, and water insoluble polysaccharide production. conclusions: the current study findings improved the understanding of c-di-gmp in e. faecalis and presented a primary study on dr75-rs11090 promoting mechanism.