Author/Authors :
Ayatollahi, Hossein Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Rahimi, Hossein Department of Internal Medicine - Mashhad University of Medical sciences, Mashhad, Iran , Sadeghian, Mohammad Hadi Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Keramati, Mohammad Reza Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Amirpour, Mojgan Mashhad University of Medical sciences, Mashhad, Iran , Sheikhi, Maryam Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Amini, Nafiseh Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Shakeri, Sepideh Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Shams, Fateme Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Zafari, Zahra Cancer Molecular Pathology Research Center - Mashhad University of Medical sciences, Mashhad, Iran , Azarkerdar, Somaieh Mashhad University of Medical sciences, Mashhad, Iran
Abstract :
Introduction: Erythroblast transformation specific Related Gene (ERG) is a member of ETS
transcription factors family, which act as a regulator of primary hematopoietic cell
differentiation. Previous researches suggested proto-oncogene for response to mitogenic
signal of different cell with MAP kinase and help to tumorgenesis, apoptotic and prognostic
role for ERG gene in acute myeloid leukemia (AML) patients. The aim of his study was to
evaluate ERG gene expression level in AML cases compare to control group.
Materials and methods: For analysis of gene expression, total RNA was isolated from bone
marrow (BM) and peripheral blood of AML `s patient. This study was conducted on 54 AML
patients and 54 healthy individuals as control group. ERG gene expression and reference
gene glucose-6-phosphate isomerase (GPI) was assessed by using real-time polymerase chain
reaction (RT-PCR) method. Clinical and laboratory findings studied cases were extracted
from medical documents.
Results: This study results indicated that the level of ERG gene expression in AML patients
had no significant differences compare to control group (1.81±2.41) (P <0.05), Also ERG
gene expression was correlated to patients hematocrit (P <0.05).
Conclusion: This research recommends that there were no significant relation between ERG
expression and control groups. It seems that ERG gene assessment is not a good index for
AML patient’s evaluation.
