Curcumin Inhibited Endothelin-1 mRNA Expression Induced by TGF- in Bovine Aortic Endothelial Cell

objectives: curcumin is a plant polyphenol compound used as a traditional supplement in many countries. the potential therapeutic or preventive effects of curcumin may be related to its antioxidant and anti-inflammatory properties. the current in vitro study aimed to investigate the effect of curcumin on the expression of endothelin-1 (et-1) stimulated by transforming growth factorbeta (tgf-β ) in bovine aortic endothelial cells (baecs). methods: bovine aortic endothelial cells derived from bovine aorta were maintained in low glucose dmem (dulbecco’s modified eagle’s medium). the time-course and different concentrations of tgf-β (2 ng/ml and 10 ng/ml) were used to evaluate et-1 mrna expression. also, the baecs were treated with 10µmof sb431542 (chemical inhibitor of tgf-β receptor) as positive control and different doses of curcumin (5 µm, 10 µm, and 15 µm). the expression of et-1 mrna was quantified by quantitative real-time polymerase chain reaction (qpcr). statistical analysis was performed by one-way anova with spss software. results: et-1 mrna expression significantly increased in six hours in the tgf-β -treated group (2.79 0.9). sb431542, as well as curcumin 10 mand 15 msignificantly decreased the expression of et-1mrna by 2.3±0.15, 1.5±0.16, and 1.02±0.01, respectively. conclusions: curcumin downregulated et-1 mrna; this result suggested a possible underlying molecular mechanism mediated through et-1 to exert its anti-inflammatory and antioxidant properties.