Introducing tumor necrosis factor as a prominent player in celiac disease and type 1 diabetes mellitus

Aim: This study aimed to screen the common genes between celiac disease (CD) and type 1 diabetes mellitus to find critical ones. Background: Celiac disease is a chronic autoimmune disorder which is correlated to type 1 diabetes mellitus (T1DM) in several molecular pathways. Understanding the clear common molecular mechanism of both diseases is of interest to scientists. Methods: The related genes to the CD and T1DM were obtained from disease query of STRING and included in two separated PPI networks by Cytoscape software version 3.7.1. The networks were analyzed by network analyzer and the hub nodes were determined. The common hubs between the two networks were selected for further analysis and enriched via gene ontology using ClueGO plugin of Cytoscape software. Also, an action map was provided by Cluepedia application of Cytoscape software. Results: Two separated networks of 2000 and 430 genes were constructed related to T1DM and CD, respectively. A total of 84 and 28 hubs were determined for T1DM and CD, respectively. There were 11 common hubs between the two networks. The first top hubs of Type 1 Diabetes Mellitus and CD networks were insulin (INS) and tumor necrosis factor (TNF), respectively. Also, 77 biological terms and pathways (in five clusters) were related to the common hubs. Action map revealed a close relationship between hubs. Conclusion: The result of this study indicated that TNF is key mediator of immune reactions in celiac disease and type 1 diabetes mellitus. Keywords: Celiac disease, Type 1 diabetes mellitus, TNF, Insulin. (Please cite as: Rezaei-Tavirani S, Rostami-Nejad M, Vafaee R, Khalkhal E, Keramatinia AA, Ehsani-Ardakani MJ, et al. Introducing tumor necrosis factor as a prominent player in celiac disease and type 1 diabetes mellitus. Gastroenterol Hepatol Bed Bench 2019;12(Suppl.1):S123-S129).