Author/Authors :
Mehrabi Pour, Mahsa Department of Biology - Islamic Azad University - Arsanjan Branch, Arsanjan, Iran , Nasiri, Mahboobeh Department of Biology - Islamic Azad University - Arsanjan Branch, Arsanjan, Iran , Kamfiroozie, Hajar Cardiovascular Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Zibaeenezhad, Mohammad Javad Cardiovascular Research Center - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Introduction: Endothelial nitric oxide synthase (eNOS), the main regulator of cardiac cell
functioning, is regulated post-transcriptionally by autophagy-related 9B (ATG9B) gene. The
proper function of the heart is partly determined by the intact interaction of these molecules. The
present study aimed to investigate the effects of ATG9B rs2373929 and rs7830 gene polymorphisms
on the predisposition to coronary artery disease (CAD).
Methods: In this hospital-based case-control study, 150 patients with CAD compared with 150
healthy subjects for the genotype distributions of rs2373929 and rs7830 polymorphisms using
T-ARMS PCR and ARMS PCR, respectively.
Results: Considering rs2373929 polymorphism, increased risk of CAD observed in the presence
of TT genotype (OR: 3.65; 95% CI: 1.77-7.53; P < 0.001) and also in the recessive model for T
allele (OR: 3.41; 95% CI: 1.76- 6.60; P < 0.001). The frequency of the T allele was higher in cases
compared to controls (OR: 1.71; 95% CI: 1.24-2.28; P = 0.001). The genotype and allele frequencies
of the rs7830 polymorphism did not differ between the two study groups.
Conclusion: The ATG9B gene rs2373929 polymorphism might involve in the pathogenesis of
the CAD and can be considered as a screening molecular marker in the subjects prone to CAD.
