Author/Authors :
Kolivand, Sedighe Department of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , Amini, Peyman Department of Radiology - Faculty of Paramedical - Tehran University of Medical Sciences, Tehran, Iran , Saffar, Hana Clinical and Anatomical Pathologist at Tehran University of Medical Science - Imam Khomeini Hospital Complex, Tehran, Iran , Rezapoor, Saeed Department of Radiology - Faculty of Paramedical - Tehran University of Medical Sciences, Tehran, Iran , Najafi, Masoud Radiology and Nuclear Medicine Department - School of Paramedical Sciences - Kermanshah University of Medical Sciences, Kermanshah, Iran , Motevaseli, Elahe Department of Molecular Medicine, School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , Nouruzi, Farzad Department of Medical Radiation Engineering - Science and Research Branch Islamic Azad University, Tehran, Iran , Shabeeb, Dheyauldeen Department of Physiology - College of Medicine - University of Misan, Misan, Iraq , Eleojo Musa, Ahmed Research Center for Molecular and Cellular Imaging - Tehran University of Medical Sciences (International Campus), Tehran, Iran
Abstract :
Introduction: Redox interactions play a key role in radiation injury including heart diseases.
In present study, we aimed to detect the possible protective role of selenium-L-methionine on
infiltration of immune cells and Duox1&2 upregulation in rat’s heart tissues.
Methods: In this study, 20 rats were divided into 4 groups (5 rats in each) namely: irradiation;
irradiation plus Selenium-L-methionine; control; and Selenium-L-methionine treatment.
Irradiation (15 Gy to chest) was performed using a cobalt-60 gamma ray source while 4 mg/kg of
selenium-L-methionine was administered intraperitoneally. Ten weeks after irradiation, rats were
sacrificed for detection of IL-4 and IL-13 cytokines, infiltration of macrophages and lymphocytes
as well as the expressions of IL4Ra1, Duox1, IL13Ra2 and Duox2.
Results: Results showed an increase in the level of IL-4 as well as the expressions of IL4Ra1, Duox1
and Duox2. Similarly, there was an increase in the infiltration of lymphocytes and macrophages.
There was significant attenuation of all these changes following treatment with selenium-Lmethionine.
Conclusion: Selenium-L-methionine has the potential to protect heart tissues against radiation
injury. Downregulation of pro-oxidant genes and modulation of some cytokines such as IL-4 are
involved in the radioprotective effect of selenium-L-methionine on heart tissues.
Keywords :
Radiation , Selenium-L-Methionine , Heart , IL-4 , Duox1 , Duox2
