Author/Authors :
Khoshsirat, Shahrokh Hearing Disorders Research Center - Loghman Hakim Hospital - Shahid Beheshti University of Medical Sciences, Tehran , Abbaszadeh, Hojjat Allah Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Khoramgah, Maryam Sadat Department of Biotechnology - School of Advanced Technologies in Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Darabi, Shahram Cellular and Molecular Research Center - Qazvin University of Medical Science , Mansouri, Vahid Faculty of Paramedical Science - Proteomics Research Center - Shahid Beheshti University of Medical Sciences,Tehran , Ahmady-Roozbahany, Navid G. Raymond Chang School - Ryerson University - Toronto, Canada , Ahrabi, Behnaz Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Bahrami, Maryam Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Vafaei-Nezhad, Saeed Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Tahmasebinia, Foozhan Department of Biological Sciences - Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan , Poor Hassan, Mahnaz Laser Application in Medical Sciences Research Center - Shahid Beheshti University of Medical Sciences, Tehran - Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Introduction: Bone marrow stromal stem cells (BMSCs), a type of adult stem cells, secrete bioactive molecules such as trophic factors, growth factors, chemokine and cytokines that may be effective against oxidative stress in neurodegenerative diseases.
In this study, we examined the protective effect of BMSCs conditioned media (CM) and photobiomodulation therapy (PBMT) on PC12 cells exposed to H2O2 as an oxidative injury model.
Methods: BMSCs were cultured and confirmed by flow cytometry analysis and underwent osteogenic and adipogenic differentiation. Then, PC12-H2O2 cells were co-treated with BMSCs-CM and PBMT. The effect of BMSCs-CM and PBMT (He-Ne laser, 632.8 nm, 3 mW, 1.2 J/cm2, 378 s) on Bax/Bcl2 expression, cell viability, was assessed by real-time PCR and MTT assay. The length of the Neurite and cell body areas were assessed by Cell A software.
Results: Flowcytometry analysis, as well as osteogenic and adipogenic staining, confirmed the BMSCs. The length of the Neurite was the highest in the group which received CM+PBMT and cell body areas were significant in CM+PBMT compared to other groups. Based on our results, elevating H2O2 concentration increased cell death significantly and using concentrations of 250 μM resulted in a dramatic increase in the mortality compared to the other groups.
Conclusion: Our result demonstrated that the combination of CM +PBMT has a protective effect on PC12 cells against oxidative stress.
Keywords :
BMSCs , PC12 cells , Hydrogen peroxide , PBMT
