Author/Authors :
Shirkani, Afshin Allergy and Clinical immunology Department - School of Medicine - Bushehr University of Medical Science, Iran , Mansouri, Atena Nanotechnology Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences, Iran , Farid Hosseini, Reza Allergy Research Center - School of Medicine - Mashhad University of Medical Sciences, Iran , Jabbari Azad, Farahzad Allergy Research Center - School of Medicine - Mashhad University of Medical Sciences, Iran , Alsadat Mahmoudian, Reihaneh Immunology Research Center - Mashhad University of medical Sciences, Iran , Montazer, Mehdi Department of Pathology - Shiraz University of Medical Sciences, Iran , Samimi, Abdolreza Department of Anesthesiologie and prehospital emergency and intensivmedicine and pain - St. Et. Elisabeth Hospital Dorsten,KKRN, Westfalen Wilhelm University Munster, Germany , Momtazi-Borojeni, AmirAbbas Department of Medical Biotechnology - Student Research Committee - Faculty of Medicine - Mashhad Universityof Medical Sciences, Iran , Abbaszadegan, Mohammad Reza Medical Genetics Research Center - Faculty of Medical Sciences -Mashhad University of Medical Sciences, Iran , Gholamin, Mehran Department of Laboratory Sciences - School of Paramedical Sciences - Mashhad University of Medical Sciences, Iran
Abstract :
Background: Allergic Rhinitis (AR) is an IgE-mediated inflammatory disorder with high morbidity rates. The
eitiology of this disease is understood to occur from a complex interaction between genetic and environmental
factors. T helper type 2 cells have been shown to have a crucial role in atopic disease due to their production of
the cytokines, intelukin (IL)-13 and IL-4, involved in inflammation. Research has shown single nucleotide
polymorphisms (SNP) of the IL-13 and IL-4 genes to be associated increased levels of IgE and with allergic
diseases such as, allergic rhinitis, asthma, and atopic dermatitis. Specifically, the rs2243250 SNP of IL-4 and
the rs20541 SNP of IL-13 have been shown to be associated with AR.
Methods: A case-control study was designed to investigate the relationship between the two SNPs rs2243250 and
rs20541 with the incidence of AR. The SNPs were examined in patients with AR and healthy controls (86 patients
and 86 controls). Blood samples were collected and DNA was extracted to evaluate the SNPs by RFLP-PCR.
Results:Recessive analysis model of the IL-13 gene (GG vs. AA+AG) revealed that the GG genotype was
more common in AR patients (P=0.36) )OR=0.8 [81% CI 0.38-1.6]). For the IL-4 gene (TC vs. TT+CC), the
TC genotype was more common in AR patients (P = 0.0022)) OR=0.71 [60% CI 1.41-5.02]). Furthermore, in
the IL-4 gene, the 590 T>C polymorphism had a significant association with AR. However, no association was
found between AR and the IL-13 rs20541 polymorphism.
Conclusions: Our findings suggest that the IL-13 polymorphism (rs20541, Exo 4, G>A, Arg130Gln) and IL-4
polymorphism (rs2243250= C-590T, promoter, T>C) are co-associated with AR and sensitivity to
aeroallergens. However, this study used a cohort of AR patients and healthy controls from the northeast of Iran.
Given the influence of ethnicity and environment on genetics, further investigation is needed to elucidate the
role of SNPs in IL-4 and IL-13 in AR among different populations.
Keywords :
Allergic rhinitis , Interleukin 4 , Interleukin 13 , Single nucleotide polymorphism
